Açaí (Euterpe oleraceae Mart.) berry extract exerts neuroprotective effects against β-amyloid exposure in vitro

Neurosci Lett. 2013 Nov 27;556:221-6. doi: 10.1016/j.neulet.2013.10.027. Epub 2013 Oct 22.

Abstract

The native South American palm açaí berry (Euterpe oleraceae Mart.) has high polyphenolic and antioxidant levels. This study examined whether açaí berry extract afforded protection against β-amyloid (Aβ)-mediated loss of cell viability and oxidative stress associated with anti-fibrillar effects. PC12 cells were exposed to either Aβ1-42, Aβ25-35 or tert butyl hydroperoxide (t-BHP), alone or in the presence of açaí extract (0.5-50μg/ml). Thioflavin T (ThT) binding assay and transmission electron microscopy were used to determine effects of açaí extract on Aβ1-42 fibril morphology and compared to açaí phenolics gallic acid, cyanidin rutinoside and cyanidin glucoside. Exposure to Aβ1-42, Aβ25-35 or t-BHP decreased PC12 cell viability. Pretreatment with açaí extract significantly improved cell viability following Aβ1-42 exposure, however Aβ25-35 or t-BHP-mediated viability loss was unaltered. Açaí extract inhibited ThT fluorescence and disrupted Aβ1-42 fibril and aggregate morphology. In comparison with other phenolics, açaí was most effective at inhibiting Aβ1-42 aggregation. Inhibition of β-amyloid aggregation may underlie a neuroprotective effect of açaí.

Keywords: Amyloid-β; Açaí; Cyanidin glucoside; Cyanidin rutinoside; Gallic acid; Oxidative stress.

MeSH terms

  • Amyloid / metabolism*
  • Amyloid / toxicity
  • Amyloid beta-Peptides / metabolism*
  • Amyloid beta-Peptides / toxicity
  • Animals
  • Anthocyanins / pharmacology
  • Arecaceae / chemistry*
  • Glucosides / pharmacology
  • Humans
  • Neurons / cytology
  • Neurons / drug effects
  • Neuroprotective Agents / pharmacology*
  • Oxidants / toxicity
  • PC12 Cells
  • Peptide Fragments / metabolism*
  • Peptide Fragments / toxicity
  • Plant Extracts / pharmacology*
  • Polyphenols / chemistry
  • Rats
  • tert-Butylhydroperoxide / toxicity

Substances

  • Amyloid
  • Amyloid beta-Peptides
  • Anthocyanins
  • Glucosides
  • Neuroprotective Agents
  • Oxidants
  • Peptide Fragments
  • Plant Extracts
  • Polyphenols
  • amyloid beta-protein (1-42)
  • amyloid beta-protein (25-36)
  • cyanidin-3-O-beta-glucopyranoside
  • tert-Butylhydroperoxide
  • cyanidin 3-rutinoside