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. 2013 Jan;45(1):329-332.
doi: 10.1002/sia.5084.

Characterization of Individual Nano-Objects With nanoprojectile-SIMS

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Free PMC article

Characterization of Individual Nano-Objects With nanoprojectile-SIMS

C-K Liang et al. Surf Interface Anal. .
Free PMC article

Abstract

Secondary ion mass spectrometry (SIMS) applied in the event-by-event bombardment/detection mode is uniquely suited for the characterization of individual nano-objects. In this approach, nano-objects are examined one-by-one, allowing for the detection of variations in composition. The validity of the analysis depends upon the ability to physically isolate the nano-objects on a chemically inert support. This requirement can be realized by deposition of the nano-objects on a Nano-Assisted Laser Desorption/Ionization (NALDI™) plate. The featured nanostructured surface provides a support where nano-objects can be isolated if the deposition is performed at a proper concentration. We demonstrate the characterization of individual nano-objects on a NALDI™ plate for two different types of nanometric bacteriophages: Qβ and M13. Scanning electron microscope (SEM) images verified that the integrity of the phages is preserved on the NALDI™ substrate. Mass spectrometric data show secondary ions from the phages are identified and resolved from those from the underlying substrate.

Keywords: NALDI™; ToF-SIMS; event-by-event SIMS analysis; gold clusters; nano-objects.

Figures

Figure 1
Figure 1
SEM images of the a) spherical Qβ phages and b) filamentous M13 phages drop-casted onto a NALDI™ plate (scale bar = 100 nm).
Figure 2
Figure 2
Negative ion mass spectrum of the Qβ phages on a NALDI™ plate. Intensities are normalized to the number of projectile impacts (~8 × 106).
Figure 3
Figure 3
a) Total mass spectrum of the Qβ phages on a NALDI™ plate. b) Coincidence mass spectrum of ions co-emitted with [Adenine-H] from the phages. c) Coincidence/anticoincidence mass spectrum constituted by events containing [Adenine-H] but without F. Coincidence and coincidence/anticoincidence mass spectra are normalized to the corresponding numbers of events.
Figure 4
Figure 4
a) Total mass spectrum of the M13 phages on a NALDI™ plate. b) Coincidence mass spectrum of ions co-emitted with [Adenine-H] from the phages. c) Coincidence/anticoincidence mass spectrum constituted by events containing [Adenine-H] but without F.

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