Expression During Embryogenesis of a Mouse Gene With Sequence Homology to the Drosophila Engrailed Gene

Cell. 1985 Nov;43(1):29-37. doi: 10.1016/0092-8674(85)90009-1.

Abstract

Regions of the mouse and human genomes with strong homology to the Drosophila engrailed gene have been identified by Southern blot analysis. One mouse engrailed-like region, Mo-en.1, has been cloned and partially sequenced; homology with the engrailed gene is localized to a 180 bp engrailed-like homeo box and 63 nucleotides immediately 3' to it. The protein sequence this region can encode includes 81 amino acids, of which 60 (75%) are identical with those of the putative translation product of the corresponding engrailed sequence. These data suggest that Mo-en.1 represents a mouse homolog of a gene of the Drosophila engrailed gene complex. Mo-en.1 has been mapped to chromosome 1, indicating it is not linked to other homeo box sequences that have been mapped in the mouse genome. Analysis of poly(A)+ RNA extracted from teratocarcinoma cells and whole mouse embryos demonstrates that the conserved homeo box region of Mo-en.1 is expressed differentially during mouse embryogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular
  • Drosophila melanogaster / genetics*
  • Embryo, Mammalian / physiology*
  • Embryo, Nonmammalian*
  • Gene Expression Regulation
  • Genes*
  • Gestational Age
  • Humans
  • Mice
  • Morphogenesis*
  • Nucleic Acid Hybridization
  • Poly A / analysis
  • RNA / analysis
  • RNA, Messenger
  • Sequence Homology, Nucleic Acid
  • Stem Cells
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Poly A
  • RNA

Associated data

  • GENBANK/M11987
  • GENBANK/Y00201