ras proteins can induce meiosis in Xenopus oocytes

Cell. 1985 Dec;43(3 Pt 2):615-21. doi: 10.1016/0092-8674(85)90233-8.

Abstract

Injection of human H-ras protein induces maturation of Xenopus oocytes; that is, progression from prophase to metaphase of meiosis. The oncogenic protein encoded by H-rasval12 is nearly a 100-fold more potent than the protein encoded by the wild-type gene. We do not observe any measurable increase or decrease in cyclic AMP concentration in injected oocytes, and the effects of H-ras protein are only partially blocked by cholera toxin. Our results suggest that not all, if any, of the effects of H-rasval12 protein in this system are mediated by adenylate cyclase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Adenylyl Cyclases / metabolism
  • Animals
  • Cholera Toxin / pharmacology
  • Cyclic AMP / physiology
  • Female
  • Guanosine Triphosphate / physiology
  • Humans
  • Meiosis*
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / physiology*
  • Oocytes / enzymology
  • Oocytes / physiology*
  • Oogenesis / drug effects
  • Progesterone / pharmacology
  • Proto-Oncogene Proteins p21(ras)
  • Xenopus laevis

Substances

  • Neoplasm Proteins
  • Progesterone
  • Guanosine Triphosphate
  • Cholera Toxin
  • Cyclic AMP
  • HRAS protein, human
  • Proto-Oncogene Proteins p21(ras)
  • Adenylyl Cyclases
  • 1-Methyl-3-isobutylxanthine