Abstract
Injection of human H-ras protein induces maturation of Xenopus oocytes; that is, progression from prophase to metaphase of meiosis. The oncogenic protein encoded by H-rasval12 is nearly a 100-fold more potent than the protein encoded by the wild-type gene. We do not observe any measurable increase or decrease in cyclic AMP concentration in injected oocytes, and the effects of H-ras protein are only partially blocked by cholera toxin. Our results suggest that not all, if any, of the effects of H-rasval12 protein in this system are mediated by adenylate cyclase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Methyl-3-isobutylxanthine / pharmacology
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Adenylyl Cyclases / metabolism
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Animals
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Cholera Toxin / pharmacology
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Cyclic AMP / physiology
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Female
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Guanosine Triphosphate / physiology
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Humans
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Meiosis*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / physiology*
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Oocytes / enzymology
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Oocytes / physiology*
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Oogenesis / drug effects
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Progesterone / pharmacology
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Proto-Oncogene Proteins p21(ras)
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Xenopus laevis
Substances
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Neoplasm Proteins
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Progesterone
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Guanosine Triphosphate
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Cholera Toxin
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Cyclic AMP
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HRAS protein, human
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Proto-Oncogene Proteins p21(ras)
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Adenylyl Cyclases
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1-Methyl-3-isobutylxanthine