Participation of voltage-dependent calcium channels in the regulation of adrenal glomerulosa function by angiotensin II and potassium

Endocrinology. 1986 Jan;118(1):112-8. doi: 10.1210/endo-118-1-112.


The stimulation of aldosterone secretion from adrenal glomerulosa cells by angiotensin II (AII), potassium, and ACTH is highly dependent on the extracellular calcium concentration. To evaluate the role of voltage-dependent calcium channels in aldosterone production, we analyzed the actions and binding of calcium channel antagonists in collagenase-dispersed adrenal glomerulosa cells and membrane-rich particles. In rat glomerulosa cells, nifedipine caused dose-dependent inhibition of the aldosterone responses to AII and potassium, with half-maximum inhibitory concentration (IC50) of 100 nM, but had no effect on ACTH or 8-bromo-cAMP stimulated steroidogenesis in adrenal glomerulosa and fasciculata cells. Binding studies with [3H]nitrendipine in adrenal glomerulosa cells revealed a high affinity site with dissociation constant (Kd) of 0.4 +/- 0.1 nM, similar to that described in other tissues but about 100-fold lower than the IC50 for blockade of aldosterone production. However, Scatchard analysis of binding data from three of seven experiments in isolated adrenal glomerulosa cells revealed a low affinity site with Kd of 130 nM, in agreement with the IC50 for the effect of nifedipine on aldosterone production. In rat adrenal particles, nitrendipine-binding sites were located in the adrenal capsule and medulla and were undetectable in the zona fasciculata. Furthermore, there was a close correlation (r = 0.92) between the concentrations of nitrendipine-binding sites and AII receptors in the different zones of the adrenal in rat, dog, and cow, suggesting a functional relationship between AII receptors and calcium channels. These studies have shown a major and selective role of voltage-dependent calcium channels in the control of aldosterone secretion by the major physiological regulators, AII and potassium.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Adrenal Glands / drug effects
  • Adrenal Glands / physiology*
  • Adrenocorticotropic Hormone / pharmacology
  • Aldosterone / biosynthesis*
  • Angiotensin II / pharmacology*
  • Animals
  • Binding, Competitive
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Cell Membrane / metabolism
  • Corticosterone / biosynthesis
  • Diltiazem / pharmacology
  • Dose-Response Relationship, Drug
  • Ion Channels / physiology*
  • Male
  • Nifedipine / analogs & derivatives
  • Nifedipine / metabolism
  • Nifedipine / pharmacology
  • Nitrendipine
  • Potassium / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Angiotensin / metabolism


  • Ion Channels
  • Receptors, Angiotensin
  • Angiotensin II
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Aldosterone
  • Adrenocorticotropic Hormone
  • Nitrendipine
  • Diltiazem
  • Nifedipine
  • Potassium
  • Calcium
  • Corticosterone