A chemical perspective on transcriptional fidelity: dominant contributions of sugar integrity revealed by unlocked nucleic acids

Angew Chem Int Ed Engl. 2013 Nov 18;52(47):12341-5. doi: 10.1002/anie.201307661. Epub 2013 Oct 24.

Abstract

Transcription unlocked: A synthetic chemical biology approach involving unlocked nucleic acids was used to dissect the contribution of sugar backbone integrity to the RNA Polymerase II (Pol II) transcription process. An unexpected dominant role for sugar-ring integrity in Pol II transcriptional efficiency and fidelity was revealed.

Keywords: RNA polymerase II; nucleic acid analogues; sugar integrity; synthetic biology; transcriptional fidelity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocatalysis
  • Carbohydrates / chemistry*
  • Kinetics
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism*
  • RNA Polymerase II / chemistry
  • RNA Polymerase II / genetics
  • RNA Polymerase II / metabolism*
  • Substrate Specificity
  • Transcription, Genetic*

Substances

  • Carbohydrates
  • Oligonucleotides
  • locked nucleic acid
  • RNA Polymerase II