Multiple sequence alignment with DIALIGN

Methods Mol Biol. 2014;1079:191-202. doi: 10.1007/978-1-62703-646-7_12.


DIALIGN is a software tool for multiple sequence alignment by combining global and local alignment features. It composes multiple alignments from local pairwise sequence similarities. This approach is particularly useful to discover conserved functional regions in sequences that share only local homologies but are otherwise unrelated. An anchoring option allows to use external information and expert knowledge in addition to primary-sequence similarity alone. The latest version of DIALIGN optionally uses matches to the PFAM database to detect weak homologies. Various versions of the program are available through Göttingen Bioinformatics Compute Server (GOBICS) at

MeSH terms

  • Computational Biology / methods*
  • Internet
  • Proteins / chemistry
  • Sequence Alignment / methods*
  • Software*
  • User-Computer Interface


  • Proteins