Cobalt ions block L-glutamate and L-aspartate-induced currents in cultured neurons from embryonic chick spinal cord

Neurosci Lett. 1985 Nov 11;61(3):345-50. doi: 10.1016/0304-3940(85)90488-4.


The effects of Co2+ on L-glutamate and L-aspartate responses wee studied in cultured spinal cord neurons of the embryonic spinal cord of the chick by employing the patch-clamp technique in whole cell mode [9]. It was found that Co2+ blocks the responses at negative membrane potentials for both amino acids, while only partial inhibition was observed at positive membrane potentials. Co2+ alone decreases the resting membrane current which exhibits reversal close to zero. It is suggested that the effects of Co2+ are produced by non-specific interaction with negative charges on the outer side of the membrane.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Aspartic Acid / antagonists & inhibitors*
  • Cell Membrane Permeability / drug effects
  • Cells, Cultured
  • Chick Embryo
  • Cobalt / pharmacology*
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Excitatory Amino Acid Antagonists*
  • Glutamates / metabolism
  • Glutamic Acid
  • Ion Channels / drug effects*
  • Membrane Potentials / drug effects
  • Spinal Cord / drug effects*


  • Excitatory Amino Acid Antagonists
  • Glutamates
  • Ion Channels
  • Aspartic Acid
  • Cobalt
  • Glutamic Acid