The nucleotide sequence in three regions of the mitochondrial plasmid (p1DNA) of Podospora anserina has been determined for almost 1,000 by of the altogether 2.4 kb. The first region beginning around excision point E1 comprises 652 by and is characterized by an AT content of about 58%. A long open reading frame (URF) (555 bp) can be attributed to this region coding for an unidentified polypeptide. No reading frame could be attributed to the second region (247 bp), since in all 6 possible phases numerous termination codons were detected. Its AT content was found to be 71%. The third region (87 bp) which ends with excision point E2 is too small for further analysis.From these data it follows that the p1DNA consists of two regions with different functions at least. However, only the larger one beginning near excision point E1 appears to code for a protein.