The need for transparency and good practices in the qPCR literature

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

MeSH terms

  • Data Collection
  • Information Services*
  • Polymerase Chain Reaction / methods*