Effects of brussels sprouts and their phytochemical components on oxidative stress-induced neuronal damages in PC12 cells and ICR mice

J Med Food. 2013 Nov;16(11):1057-61. doi: 10.1089/jmf.2012.0280. Epub 2013 Oct 31.


In this study, the protective effects of Brussels sprouts extract and its major constituents against oxidative stress-induced damages were investigated in rat pheochromocytoma cells and Institute of Cancer Research mice. The major constituents of Brussels sprouts (3,4',5,7-tetrahydroxyflavone (kaempferol), indole-3-carbinol, and phenethyl isothiocyanate) were selectively tested. Of these, the flavonoid compound, kaempferol exhibited the highest potency in radical scavenging activity (1,1-diphenyl-2-picryl hydrazyl assay and oxygen radical absorbance capacity assay) and was most protective against oxidative stress in neuronal cell assays (measurement of intracellular oxidative stress levels and cell viability). In mice, after 4 weeks of kaempferol administration, significant protection against amyloid beta (Aβ) peptide-induced neurotoxicity was also observed, as assessed through the passive avoidance test. Taken together, the results suggest that Brussels sprouts could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its major constituent, kaempferol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy
  • Alzheimer Disease / metabolism*
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Antioxidants / pharmacology*
  • Biphenyl Compounds / metabolism
  • Brain / cytology
  • Brain / drug effects*
  • Brain / metabolism
  • Brassica / chemistry*
  • Cell Survival / drug effects
  • Indoles / pharmacology
  • Isothiocyanates / pharmacology
  • Kaempferols / pharmacology*
  • Mice
  • Mice, Inbred ICR
  • Neuroprotective Agents / pharmacology
  • Neurotoxicity Syndromes / drug therapy
  • Neurotoxicity Syndromes / metabolism
  • Oxidative Stress / drug effects*
  • PC12 Cells
  • Picrates / metabolism
  • Plant Extracts / pharmacology
  • Rats


  • Amyloid beta-Peptides
  • Antioxidants
  • Biphenyl Compounds
  • Indoles
  • Isothiocyanates
  • Kaempferols
  • Neuroprotective Agents
  • Picrates
  • Plant Extracts
  • phenethyl isothiocyanate
  • kaempferol
  • indole-3-carbinol
  • 1,1-diphenyl-2-picrylhydrazyl