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. 2013 Nov 1;38(21):4510-3.
doi: 10.1364/ol.38.004510.

Resolution and Contrast Enhancement in Coherent anti-Stokes Raman-scattering Microscopy

Resolution and Contrast Enhancement in Coherent anti-Stokes Raman-scattering Microscopy

Alicja Gasecka et al. Opt Lett. .

Abstract

We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λ(p) is achieved.

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