Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

Opt Lett. 2013 Nov 1;38(21):4510-3. doi: 10.1364/ol.38.004510.


We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36λ(p) is achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Stem / cytology
  • Lasers
  • Mice
  • Microscopy / methods*
  • Myelin Sheath / metabolism
  • Optical Phenomena
  • Spectrum Analysis, Raman*