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, 16 (3-4), 163-6

Microspore Culture of Radish (Raphanus Sativus L.): Influence of Genotype and Culture Conditions on Embryogenesis

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Microspore Culture of Radish (Raphanus Sativus L.): Influence of Genotype and Culture Conditions on Embryogenesis

Y Takahata et al. Plant Cell Rep.

Abstract

A number of factors influencing embryogenesis from isolated microspores of radish (Raphanus sativus) were examined. Of 11 genotypes evaluated, six produced embryos ranging from 8.3 embryos per 10(5) microspores for 'Chugoku-ao' to 0.2 for 'Tenshun', but five genotypes were not responsive. An initial culture period at elevated temperature before incubation at 25°C was essential for induction of microspore embryogenesis. However, the optimum period of the treatment varied among genotypes and/or experiments. Bud size also influenced microspore embryogenesis. Though optimum bud size was different between genotypes, the microspore populations represented in these buds contained uninucleate and binucleate microspores. Selection of embryogenic microspores using percoll density gradient resulted in up to 1.3-fold increase of embryo yield. Though almost all embryos failed to develop directly into plantlets, plants were obtained by multiple subcultures. The regenerated plants had hyperploid chromosome numbers.

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References

    1. Plant Cell Rep. 1992 May;11(4):215-8 - PubMed
    1. Plant Cell Rep. 1993 Jul;12(9):496-500 - PubMed
    1. Plant Cell Rep. 1995 Jun;14(9):580-4 - PubMed
    1. Plant Cell Rep. 1989 Dec;8(8):486-8 - PubMed
    1. Plant Cell Rep. 1995 Jul;14(10):648-51 - PubMed
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