The CEP Family in Land Plants: Evolutionary Analyses, Expression Studies, and Role in Arabidopsis Shoot Development

J Exp Bot. 2013 Dec;64(17):5371-81. doi: 10.1093/jxb/ert331. Epub 2013 Oct 31.

Abstract

In Arabidopsis, more than 1000 putative small signalling peptides have been predicted, but very few have been functionally characterized. One class of small post-translationally modified signalling peptides is the C-TERMINALLY ENCODED PEPTIDE (CEP) family, of which one member has been shown to be involved in regulating root architecture. This work applied a bioinformatics approach to identify more members of the CEP family. It identified 10 additional members and revealed that this family only emerged in flowering plants and was absent from extant members of more primitive plants. The data suggest that the CEP proteins form two subgroups according to the CEP domain. This study further provides an overview of specific CEP expression patterns that offers a comprehensive framework to study the role of the CEP signalling peptides in plant development. For example, expression patterns point to a role in aboveground tissues which was corroborated by the analysis of transgenic lines with perturbed CEP levels. These results form the basis for further exploration of the mechanisms underlying this family of peptides and suggest their putative roles in distinct developmental events of higher plants.

Keywords: Arabidopsis; CEP expression; evolutionary analyses; phylogeny; shoot development.; small signalling peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / cytology
  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Biological Evolution
  • Embryophyta / cytology
  • Embryophyta / drug effects
  • Embryophyta / genetics
  • Embryophyta / growth & development
  • Gene Expression Regulation, Plant*
  • Magnoliopsida / cytology
  • Magnoliopsida / drug effects
  • Magnoliopsida / genetics
  • Magnoliopsida / growth & development
  • Molecular Sequence Data
  • Multigene Family
  • Peptides / genetics*
  • Peptides / metabolism
  • Phenotype
  • Phylogeny
  • Plant Growth Regulators / pharmacology
  • Plant Roots / cytology
  • Plant Roots / drug effects
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Shoots / cytology
  • Plant Shoots / drug effects
  • Plant Shoots / genetics
  • Plant Shoots / growth & development
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Protein Structure, Tertiary
  • Signal Transduction*

Substances

  • Arabidopsis Proteins
  • Peptides
  • Plant Growth Regulators