Serologic and T cell recognition of truncated transplantation antigens encoded by in vitro deleted class I major histocompatibility genes

J Immunol. 1986 Feb 15;136(4):1472-81.


Gene constructions encoding a leader sequence and the C2, transmembrane, and intracytoplasmic exons of both H-2Ld (pLd-C2) and H-2Dd (pDd-C2) were expressed as cell surface proteins when stably introduced into mouse L cells. Distinct proteins of 17.5 and 16 kd precipitated from pDd-C2 transformants reacted with a C2 domain-specific monoclonal antibody, whereas the pLd-C2 transformants expressed a single 19 kd glycoprotein. A xenoantiserum specific for the exon VI/VIII fusion peptide of H-2Dd precipitated only the 16 kd product, suggesting that this protein was derived from an alternately spliced transcript lacking exon VII of the native gene. CTL specifically recognized the pLd-C2 and pDd-C2 gene products, indicating the existence of CTL reactive against polymorphic determinants in the C2 domain of class I antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / analysis
  • Antigens, Surface / genetics
  • Chromosome Deletion*
  • DNA / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Genes, MHC Class II*
  • H-2 Antigens / analysis
  • H-2 Antigens / genetics*
  • L Cells / metabolism
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics
  • Mice
  • Mice, Inbred BALB C
  • RNA / metabolism
  • Recombination, Genetic*
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Cytotoxic / metabolism
  • Transcription, Genetic
  • Transformation, Genetic


  • Antigens, Surface
  • H-2 Antigens
  • H-2K(K) antigen
  • Membrane Proteins
  • RNA
  • DNA