Saccharomyces cerevisiae growth media

Jessica S Dymond

doi:10.1016/B978-0-12-420067-8.00012-X

Saccharomyces cerevisiae growth media

Methods Enzymol. 2013:533:191-204. doi: 10.1016/B978-0-12-420067-8.00012-X.

Author

Jessica S Dymond¹

Affiliation

¹ The High Throughput Biology Center and the Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jsiege16@jhmi.edu.

PMID: 24182924
DOI: 10.1016/B978-0-12-420067-8.00012-X

Abstract

This protocol describes the preparation of different types of media to grow the yeast Saccharomyces cerevisiae, both on plates and in liquid culture.

Keywords: Growth media; Preparation of minimal media; Preparation of sporulation media; Preparation of synthetic complete (drop-out) media; Saccharomyces cerevisiae; Yeast extract peptone, dextrose (YEPD/YED).

MeSH terms

Culture Media* / chemistry
Microbiological Techniques / instrumentation
Microbiological Techniques / methods*
Peptones
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / growth & development*
Saccharomyces cerevisiae / physiology
Spores, Fungal / growth & development

Substances

Culture Media
Peptones