Freezing tissue is a convenient method for long-term storage. In addition, sectioning tissue under frozen conditions or embedded within a frozen matrix allows for stabilization of tissue during the sectioning process. This is advantageous for small and/or delicate tissue (e.g., rodent brains younger than postnatal day 7) and tissue that will be sectioned very thin (usually <150 μm).
Keywords: Bembedding tissue sample; Cryoprotectant media; Cryostat section; Frozen tissue sections; Preparation of slides for immunochemistry.
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