Preparation of cells for microscopy using cytospin

Abstract

Microscopy is a simple, direct technique for examining the morphology of cells and their organelles. Cells are immobilized on a solid support that is optically suitable for microscopy, and then fixed. When coupled with antibody-based immunofluorescent or immunocytochemical methods, the expression of specific proteins can be quantified and localized. Specialized stains and dyes can also be used to visualize nucleic acids or other cellular structures. See alternative protocols for fixation of suspension cells on Preparation of Cells for Microscopy using 'Cell Blocks' and for adherent cells on Preparation of Cells for Microscopy using Chamber Slides and Coverslips.

Keywords: Collecting cells; Cytospin; Fixation and drying; Immunocytochemistry; Immunofluorescence.