Reverse-phase HPLC analysis and purification of small molecules

Kirstie Canene-Adams

doi:10.1016/B978-0-12-420067-8.00023-4

Reverse-phase HPLC analysis and purification of small molecules

Methods Enzymol. 2013:533:291-301. doi: 10.1016/B978-0-12-420067-8.00023-4.

Author

Kirstie Canene-Adams¹

Affiliation

¹ Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: kirstieadams26@yahoo.com.

PMID: 24182935
DOI: 10.1016/B978-0-12-420067-8.00023-4

Abstract

Reversed phase high-performance liquid chromatography (HPLC) is utilized for the separation of molecules due to their polarity in order to quantify, identify, and/or purify various samples such as those from serum, human and animal tissues, drugs, and foods. The following protocols are for extracting carotenoids from samples and subsequent HPLC analysis. If you are interested in another compound for HPLC analysis, Sigma-Aldrich® has a wonderful online resource for multiple adaptations to the HPLC protocol for the analysis of hundreds of compounds (see References).

Keywords: Extraction of carotenoids; High-performance liquid chromatography (HPLC); Purification of small molecules; Reverse-phase HPLC-PDA system; Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.

MeSH terms

Animals
Carotenoids / analysis
Carotenoids / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Chromatography, Reverse-Phase / methods*
Humans

Substances

Carotenoids