Antineoplastic activity of strawberry (Fragaria×ananassa Duch.) crude extracts on B16-F10 melanoma cells

Mol Biosyst. 2014 Jun;10(6):1255-63. doi: 10.1039/c3mb70316a. Epub 2013 Nov 4.


The antiproliferative and differentiation potential of anthocyanin-rich strawberry fruit crude extracts (SE) were investigated on B16-F10 murine melanoma cells. Treatment of melanoma cells with SE produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, and the enhancement of tissue transglutaminase (TG2, EC activity (used as a differentiation marker). To gain further insight into profiling altered protein expression as a potential biomarker of the SE action on melanoma cells, analysis of the proteomic profile was performed on the treated B16-F10 cells, compared to the control. Following SE treatment, 30 proteins resulted up-regulated, and 87 proteins were down-regulated. In particular proteins overexpressed in cancer cells, involved in tumor progression and metabolism, were down-regulated. The possibility that SE may affect the Warburg effect in B16-F10 melanoma cells is discussed.

MeSH terms

  • Animals
  • Anthocyanins / pharmacology*
  • Anthocyanins / therapeutic use
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Fragaria / chemistry*
  • GTP-Binding Proteins / metabolism
  • Gene Expression Regulation, Neoplastic / drug effects
  • Melanoma / drug therapy
  • Melanoma / pathology*
  • Mice
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Polyamines / metabolism
  • Protein Glutamine gamma Glutamyltransferase 2
  • Proteomics
  • Transglutaminases / metabolism


  • Anthocyanins
  • Antineoplastic Agents
  • Plant Extracts
  • Polyamines
  • Protein Glutamine gamma Glutamyltransferase 2
  • Transglutaminases
  • GTP-Binding Proteins