A simple procedure is developed to probe in situ the association between lipid bilayers and colloidal particles. Here, a one-step method is applied to generate giant unilamellar 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles (GUVs) by application of an alternating electric field directly in the presence of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) microgels. We demonstrate that the soft PNIPAM microgel particles act as switchable stabilizers for lipid membranes. The change of the particle conformation from the swollen to the collapsed state enables the reversible control of the microgel adsorption as a function of temperature. At 20 °C, the swollen and hydrophilic soft microgel particles adsorb evenly and densely pack in 2D hexagonal arrays at the DOPC GUV surfaces. In contrast, at 40 °C, that is, above the volume phase transition temperature (TVPT = 32 °C) of the PNIPAM microgels, the collapsed and more hydrophobic particles partially desorb and self-organize into domains at the GUV/GUV interfaces. This study shows that thermoresponsive PNIPAM microgels can be used to increase and control the stability of lipid vesicles where the softness and deformability of these types of particles play a major role. The observed self-assembly, where the organization and position of the particles on the GUV surface can be controlled "on demand", opens new routes for the design of nanostructured materials.