In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation

Hum Immunol. 1986 Jan;15(1):109-17. doi: 10.1016/0198-8859(86)90321-6.

Abstract

This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibody Formation
  • B-Lymphocytes / immunology*
  • Cell Line
  • Cell Transformation, Viral*
  • Epitopes / analysis*
  • HLA Antigens / immunology*
  • Herpesvirus 4, Human / physiology
  • Humans
  • Immunization, Secondary
  • Isoantibodies / immunology
  • Lymphocyte Activation
  • Lymphocytes / immunology

Substances

  • Epitopes
  • HLA Antigens
  • Isoantibodies