Abstract
In the generation of allogeneic, hapten-modified and virus-specific cytotoxic T cell (CTL) responses there is usually a requirement for T-T interaction between the T helper cell (TH) and the precursor CTL (CTL). We have investigated the role of a TH signal in the induction of a xenogeneic mouse antihuman CTL response by using membranes and liposomes bearing the xenogeneic antigen to stimulate primed responders. The TH signal can be achieved by either an Ia-restricted, L3T4+ DR-specific T cell or by the addition of nonspecific T helper factors(s). This signal is delivered to an Lyt-2+, L3T4-DR-specific CTL to generate active xenogeneic (xeno-) CTL. The roles of the T cell accessory molecules L3T4, Lyt-2, and LFA-1 in the generation of and target cell lysis by xeno-CTL are investigated.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Ly / immunology
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Antigens, Surface / immunology
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Cell Communication
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Cytotoxicity, Immunologic
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Epitopes / genetics
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Epitopes / immunology
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Female
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology*
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Humans
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Lymphocyte Activation*
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Lymphocyte Function-Associated Antigen-1
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Rats
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Rats, Inbred Lew
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Species Specificity
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T-Lymphocytes, Cytotoxic / classification
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Helper-Inducer / immunology*
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T-Lymphocytes, Helper-Inducer / metabolism
Substances
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Ly
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Antigens, Surface
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Epitopes
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Histocompatibility Antigens Class II
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Lymphocyte Function-Associated Antigen-1