Effect of thymoquinone on ethanol and high fat diet induced chronic pancreatitis--a dose response study in rats

Indian J Exp Biol. 2013 Apr;51(4):292-302.


A significant increase in serum lipase, amylase, capase-1 and myeloperoxidase activities, oxidative stress index (OSI), IL-1beta and IL-18 was observed in rats receiving ethanol (EtOH) and high fat diet (HFD). Thymoquinone (TQ) supplementation along with EtOH and HFD significantly decreased the levels of serum lipase, amylase, capase-1, myeloperoxidase, OSI and maintained the antioxidant status when compared to untreated EtOH and HFD fed rats. Among the 4 doses, 100 mg of TQ/kg body weight was found to provide optimum protective effect on pancreas against EtOH and HFD induced abnormal changes. Histological observations added more evidence for the anti-inflammatory effect of TQ.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Benzoquinones / pharmacology*
  • Body Weight
  • Diet, High-Fat / adverse effects*
  • Dose-Response Relationship, Drug
  • Ethanol / adverse effects*
  • Glutathione / metabolism
  • Inflammation
  • Interleukin-18 / metabolism
  • Interleukin-1beta / metabolism
  • Lipase / blood
  • Lipid Peroxides / chemistry
  • Male
  • Oxidative Stress
  • Pancreatitis, Chronic / chemically induced
  • Pancreatitis, Chronic / drug therapy*
  • Peroxidase / metabolism
  • Rats
  • Rats, Wistar


  • Antioxidants
  • Benzoquinones
  • Interleukin-18
  • Interleukin-1beta
  • Lipid Peroxides
  • Ethanol
  • Peroxidase
  • Lipase
  • Glutathione
  • thymoquinone