Taurine attenuates lung ischemia-reperfusion injury after lung transplantation in rats

J Anesth. 2014 Jun;28(3):347-53. doi: 10.1007/s00540-013-1741-2. Epub 2013 Nov 6.


Purpose: Taurine, the major intracellular free amino acid found in high concentrations in mammalian cells, is known to be an endogenous antioxidant and a membrane-stabilizing agent. It was hypothesized that taurine may be effective in reducing ischemia-reperfusion injury after lung transplantation and an experimental study was conducted in a rat model.

Methods: The number of Sprague-Dawley rats used in the study was 35. Animals were randomized into five groups of 7 rats each, including control, donor I, donor II, ischemia-reperfusion injury, and treatment groups. All animals were exposed to the same experimental conditions in the preoperative period. Rats were fixed in a supine position after the induction. After the rats were shaved, a left pneumonectomy was performed following sternotomy in control, donor I, and donor II groups. The harvested grafts in donor I and donor II groups were transplanted to the rats of the ischemia-reperfusion group and treatment group, respectively. However, taurine was administered intraperitoneally for 3 days before the harvesting procedure in donor II. All harvested lungs were kept in a Euro-Collins solution at +4 °C for 24 h in a half-inflated manner. After harvesting and transplantation, lungs were sampled for histopathological and biochemical analysis.

Results: Malondialdehyde and superoxide dismutase, glutathione peroxidase, and catalase levels were lower in the treatment group than the other groups (p < 0.05). Histopathological findings were better in treatment group than the ischemia-reperfusion group (p < 0.05).

Conclusion: It was demonstrated that donor treatment with taurine resulted in preservation of transplanted lung tissue in respect to histopathological and biochemical findings.

MeSH terms

  • Animals
  • Antioxidants / therapeutic use*
  • Disease Models, Animal
  • Hypertonic Solutions
  • Lung / blood supply
  • Lung / drug effects*
  • Lung / metabolism
  • Lung / pathology*
  • Lung Transplantation / methods
  • Male
  • Malondialdehyde / analysis
  • Malondialdehyde / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology*
  • Superoxide Dismutase / analysis
  • Superoxide Dismutase / metabolism
  • Taurine / therapeutic use*


  • Antioxidants
  • Euro-Collins' solution
  • Hypertonic Solutions
  • Taurine
  • Malondialdehyde
  • Superoxide Dismutase