Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase

Transgenic Res. 2014 Feb;23(1):177-85. doi: 10.1007/s11248-013-9764-x. Epub 2013 Nov 7.


We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alleles
  • Animals
  • Breeding
  • Embryo, Mammalian
  • Embryonic Development / genetics*
  • Embryonic Stem Cells
  • Integrases / genetics*
  • Mice
  • Mice, Transgenic


  • Cre recombinase
  • Integrases