A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Colloids Surf B Biointerfaces. 2014 Feb 1;114:225-33. doi: 10.1016/j.colsurfb.2013.10.008. Epub 2013 Oct 17.

Abstract

We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9 ng/cm(2)) compared to the bare metal surface (310 ng/cm(2)). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.

Keywords: Bioactivity; Biofunctionalization; Biomimetic; Cell response; Protein immobilization; Protein resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Alkaline Phosphatase / metabolism
  • Benzoates / chemistry
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Coated Materials, Biocompatible / pharmacology
  • Elastin / pharmacology*
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Materials Testing
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / enzymology
  • Photoelectron Spectroscopy
  • Quinolines / chemistry
  • Recombinant Proteins / pharmacology*
  • Surface Properties
  • Titanium / pharmacology*

Substances

  • Benzoates
  • Coated Materials, Biocompatible
  • Quinolines
  • Recombinant Proteins
  • 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde
  • Elastin
  • Titanium
  • L-Lactate Dehydrogenase
  • Alkaline Phosphatase