Silver Staining of Proteins in Polyacrylamide Gels: Increased Sensitivity Through a Combined Coomassie Blue-Silver Stain Procedure

Anal Biochem. 1985 Dec;151(2):466-70. doi: 10.1016/0003-2697(85)90206-4.

Abstract

A combined Coomassie blue-silver stain method has been developed in sodium dodecyl sulfate-polyacrylamide gels for the detection of proteins using the model compounds bovine serum albumin, lysozyme, and recombinant DNA-derived human insulin. Sensitivity was enhanced 2.2 to 8.6 times by the new method relative to that of silver staining alone. The new method may also be useful in enhancing detection sensitivities of other proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel / methods
  • Indicators and Reagents
  • Proteins / analysis*
  • Reagent Kits, Diagnostic
  • Rosaniline Dyes
  • Silver
  • Staining and Labeling

Substances

  • Indicators and Reagents
  • Proteins
  • Reagent Kits, Diagnostic
  • Rosaniline Dyes
  • Silver
  • coomassie Brilliant Blue