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, 11 (4), 215-8

Embryogenesis and Plant Regeneration From Isolated Microspores of Brassica Rapa L. Ssp. Oleifera

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Embryogenesis and Plant Regeneration From Isolated Microspores of Brassica Rapa L. Ssp. Oleifera

L Burnett et al. Plant Cell Rep.

Abstract

Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2-3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33-35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment.

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References

    1. Plant Cell Rep. 1987 Apr;6(2):94-7 - PubMed
    1. Planta. 1990 Apr;181(1):18-26 - PubMed
    1. Plant Cell Rep. 1989 Dec;8(8):486-8 - PubMed
    1. Theor Appl Genet. 1989 Dec;78(6):831-5 - PubMed
    1. Exp Cell Res. 1968 Apr;50(1):151-8 - PubMed
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