Coordination of cell proliferation and cell fate determination by CES-1 snail

PLoS Genet. 2013 Oct;9(10):e1003884. doi: 10.1371/journal.pgen.1003884. Epub 2013 Oct 31.

Abstract

The coordination of cell proliferation and cell fate determination is critical during development but the mechanisms through which this is accomplished are unclear. We present evidence that the Snail-related transcription factor CES-1 of Caenorhabditis elegans coordinates these processes in a specific cell lineage. CES-1 can cause loss of cell polarity in the NSM neuroblast. By repressing the transcription of the BH3-only gene egl-1, CES-1 can also suppress apoptosis in the daughters of the NSM neuroblasts. We now demonstrate that CES-1 also affects cell cycle progression in this lineage. Specifically, we found that CES-1 can repress the transcription of the cdc-25.2 gene, which encodes a Cdc25-like phosphatase, thereby enhancing the block in NSM neuroblast division caused by the partial loss of cya-1, which encodes Cyclin A. Our results indicate that CDC-25.2 and CYA-1 control specific cell divisions and that the over-expression of the ces-1 gene leads to incorrect regulation of this functional 'module'. Finally, we provide evidence that dnj-11 MIDA1 not only regulate CES-1 activity in the context of cell polarity and apoptosis but also in the context of cell cycle progression. In mammals, the over-expression of Snail-related genes has been implicated in tumorigenesis. Our findings support the notion that the oncogenic potential of Snail-related transcription factors lies in their capability to, simultaneously, affect cell cycle progression, cell polarity and apoptosis and, hence, the coordination of cell proliferation and cell fate determination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / genetics
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • Carcinogenesis / genetics
  • Cell Cycle / genetics*
  • Cell Differentiation / genetics*
  • Cell Polarity / genetics
  • Cell Proliferation / genetics*
  • Cyclin A / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism
  • Humans
  • Mammals
  • Repressor Proteins / genetics
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • cdc25 Phosphatases / genetics

Substances

  • Caenorhabditis elegans Proteins
  • Cyclin A
  • DNA-Binding Proteins
  • Dnj-11 protein, C elegans
  • EGL-1 protein, C elegans
  • Heat-Shock Proteins
  • Repressor Proteins
  • Transcription Factors
  • ces-1 protein, C elegans
  • CDC-25.1 protein, C elegans
  • cdc25 Phosphatases