Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10

Cell. 1986 Mar 28;44(6):913-23. doi: 10.1016/0092-8674(86)90014-0.


The human excision repair gene ERCC-1 was cloned after DNA mediated gene transfer to the CHO mutant 43-3B, which is sensitive to ultraviolet light and mitomycin-C. We describe the cloning and sequence analysis of the ERCC-1 cDNA and partial characterization of the gene. ERCC-1 has a size of 15 kb and is located on human chromosome 19. The ERCC-1 precursor RNA is subject to alternative splicing of an internal 72 bp coding exon. Only the cDNA of the larger 1.1 kb transcript, encoding a protein of 297 amino acids, was able to confer resistance to ultraviolet light and mitomycin-C on 43-3B cells. Significant amino acid sequence homology was found between the ERCC-1 gene product and the yeast excision repair protein RAD10. The most homologous region displayed structural homology with DNA binding domains of various polypeptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aspartate Carbamoyltransferase*
  • Base Sequence
  • Biological Evolution
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)*
  • Chromosome Deletion*
  • Chromosome Mapping
  • Cloning, Molecular*
  • Cricetinae
  • Cricetulus
  • DNA / analysis*
  • DNA Repair*
  • DNA-Binding Proteins / analysis
  • Dihydroorotase*
  • Genes*
  • Genes, Fungal*
  • Genetic Complementation Test
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Multienzyme Complexes*
  • Poly A / metabolism
  • Proteins / analysis
  • RNA / metabolism
  • RNA, Messenger
  • Saccharomyces cerevisiae / genetics*


  • CAD trifunctional enzyme
  • DNA-Binding Proteins
  • Multienzyme Complexes
  • Proteins
  • RNA, Messenger
  • Poly A
  • RNA
  • DNA
  • Aspartate Carbamoyltransferase
  • Dihydroorotase
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)

Associated data

  • GENBANK/M13194