Microarray analysis of tomato's early and late wound response reveals new regulatory targets for Leucine aminopeptidase A

PLoS One. 2013 Oct 24;8(10):e77889. doi: 10.1371/journal.pone.0077889. eCollection 2013.

Abstract

Wounding due to mechanical injury or insect feeding causes a wide array of damage to plant cells including cell disruption, desiccation, metabolite oxidation, and disruption of primary metabolism. In response, plants regulate a variety of genes and metabolic pathways to cope with injury. Tomato (Solanum lycopersicum) is a model for wound signaling but few studies have examined the comprehensive gene expression profiles in response to injury. A cross-species microarray approach using the TIGR potato 10-K cDNA array was analyzed for large-scale temporal (early and late) and spatial (locally and systemically) responses to mechanical wounding in tomato leaves. These analyses demonstrated that tomato regulates many primary and secondary metabolic pathways and this regulation is dependent on both timing and location. To determine if LAP-A, a known modulator of wound signaling, influences gene expression beyond the core of late wound-response genes, changes in RNAs from healthy and wounded Leucine aminopeptidase A-silenced (LapA-SI) and wild-type (WT) leaves were examined. While most of the changes in gene expression after wounding in LapA-SI leaves were similar to WT, overall responses were delayed in the LapA-SI leaves. Moreover, two pathogenesis-related 1 (PR-1c and PR-1a2) and two dehydrin (TAS14 and Dhn3) genes were negatively regulated by LAP-A. Collectively, this study has shown that tomato wound responses are complex and that LAP-A's role in modulation of wound responses extends beyond the well described late-wound gene core.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers / metabolism*
  • Blotting, Western
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant*
  • Leucyl Aminopeptidase / genetics*
  • Leucyl Aminopeptidase / metabolism
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / growth & development
  • Lycopersicon esculentum / metabolism*
  • Oligonucleotide Array Sequence Analysis*
  • RNA, Messenger / genetics
  • RNA, Plant / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Wound Healing*

Substances

  • Biomarkers
  • RNA, Messenger
  • RNA, Plant
  • Leucyl Aminopeptidase

Grant support

This work and JF were supported by the National Science Foundation grant (IOS 0725093) to LLW. MS was supported by supported in part by the National Science Foundation-funded ChemIGERT program (DGE 0504249) and IOS 0725093. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.