Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

FEBS Lett. 1986 Mar 31;198(2):315-20. doi: 10.1016/0014-5793(86)80428-8.


Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca2+]i). It is suggested that NGF has a unique mode of action independent of [Ca2+]i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca2+]i rise.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / analysis*
  • Dose-Response Relationship, Drug
  • Histamine Release / drug effects
  • In Vitro Techniques
  • Lysophospholipids*
  • Male
  • Mast Cells / analysis
  • Mast Cells / drug effects*
  • Nerve Growth Factors / pharmacology*
  • Phosphatidylserines / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Tetradecanoylphorbol Acetate / pharmacology


  • Lysophospholipids
  • Nerve Growth Factors
  • Phosphatidylserines
  • lysophosphatidylserine
  • Tetradecanoylphorbol Acetate
  • Calcium