Transfer of specificity by murine alpha and beta T-cell receptor genes

Nature. 1986 Mar 20-26;320(6059):232-8. doi: 10.1038/320232a0.

Abstract

T-cell receptor alpha- and beta-chain genes were isolated from a class I major histocompatibility complex-restricted cytotoxic T-cell clone and transferred by protoplast fusion into another cytolytic T-cell clone of different specificity. Expression of the transfected alpha and beta genes endowed the recipient cell with the specificity of the donor cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Cell Line
  • Clone Cells
  • DNA, Recombinant
  • Histocompatibility Antigens / immunology
  • Hybridomas / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Nucleic Acid Hybridization
  • RNA / genetics
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell / immunology
  • T-Lymphocytes, Cytotoxic / immunology
  • Transcription, Genetic
  • Transfection*

Substances

  • DNA, Recombinant
  • Histocompatibility Antigens
  • Receptors, Antigen, T-Cell
  • RNA

Associated data

  • GENBANK/X03668
  • GENBANK/X03669
  • GENBANK/X03670
  • GENBANK/X03671
  • GENBANK/X03760
  • GENBANK/X03761
  • GENBANK/X03762