Structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus

Nature. 1986 Mar 20-26;320(6059):277-80. doi: 10.1038/320277a0.

Abstract

A role for proto-oncogenes in the regulation and modulation of cell proliferation has been suggested by the findings that the B-chain of platelet-derived growth factor (PDGF) is encoded by the proto-oncogene sis and that the erb-B oncogene product is a truncated form of the epidermal growth factor (EGF) receptor. Furthermore, the product of the proto-oncogene fms (c-fms) may be related or identical to the receptor for macrophage colony-stimulating factor (CSF-1). v-fms is the transforming gene of the McDonough strain of feline sarcoma virus (SM-FeSV) and belongs to the family of src-related oncogenes which have tyrosine-specific kinase activity. Furthermore, nucleotide sequence analysis of the v-fms gene product revealed topological properties of a cell-surface receptor protein. To elucidate the features involved in the conversion of a normal cell-surface receptor gene into an oncogenic one, we have now determined the complete nucleotide sequence of a human c-fms complementary DNA. The 972-amino-acid c-fms protein has an extracellular domain, a membrane-spanning region, and a cytoplasmic tyrosine protein kinase domain. Comparison of the feline v-fms and human c-fms sequences reveals that the proteins share extensive homology but have different carboxyl termini.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA
  • ErbB Receptors
  • Female
  • Glycoproteins / genetics
  • Humans
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Placenta / analysis
  • Poly A / genetics
  • Pregnancy
  • Proto-Oncogene Proteins
  • Proto-Oncogenes*
  • RNA / genetics
  • RNA, Messenger
  • Receptor, Insulin / genetics
  • Receptors, Cell Surface / genetics*
  • Receptors, LDL / genetics
  • Retroviridae / genetics*
  • Sarcoma Viruses, Feline / genetics*

Substances

  • Glycoproteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, LDL
  • Poly A
  • RNA
  • DNA
  • ErbB Receptors
  • Receptor, Insulin

Associated data

  • GENBANK/X03663