Intrapulmonary Concentration of Levofloxacin in Patients With Idiopathic Pulmonary Fibrosis

Pulm Pharmacol Ther. 2014 Jun;28(1):49-52. doi: 10.1016/j.pupt.2013.10.004. Epub 2013 Nov 7.

Abstract

Patients with idiopathic pulmonary fibrosis (IPF) have significantly impaired pulmonary diffusion, which may affect the pulmonary concentration of many drugs, including antibiotics. In this study, we compared the difference in pulmonary levofloxacin (LVFX) concentration between patients with normal lung function and IPF. The IPF group included 10 patients with a proven diagnosis of IPF and a diffusing capacity for carbon monoxide ranging from 40% to 70% of predicted values. The control group included 10 patients with normal pulmonary function. Blood and bronchoalveolar lavage fluid (BALF) were taken at 3-3.5 h after fasting. LVFX (500 mg) was administered orally. LVFX concentrations in the serum and BALF were determined using HPLC-MS/MC. The level of LVFX in alveolar epithelial lining fluid (ELF) was calculated using the following formula: LVFX ELF = LVFX BALF × (Urea serum/Urea BALF). No significant differences in age, body weight, height, and calculated creatinine clearance and BALF retrieval rate were observed between groups. LVFX serum concentrations in the IPF and control groups were (5.97 ± 1.28) μg/ml and (6.84 ± 3.43) μg/ml, respectively (P = 0.4727). ELF concentration of LVFX in the control group was (27.81 ± 21.36) μg/ml, while the concentration in the IPF group was (10.17 ± 2.46) μg/ml, less than half of that in the controls (P = 0.0058). The intrapulmonary concentration of LVFX in IPF patients was lower than those with normal lung function. Notably, however, the ELF LVFX concentration following 500 mg once-daily exceeded the MIC90 of common respiratory pathogens. Excellent antibacterial efficacy of LVFX can be expected for IPF patients in the treatment of respiratory tract infections.

Keywords: Alveolar epithelial lining fluid; Idiopathic pulmonary fibrosis; Levofloxacin; Pharmacokinetics.

Publication types

  • Controlled Clinical Trial

MeSH terms

  • Administration, Oral
  • Adult
  • Aged
  • Anti-Bacterial Agents / pharmacokinetics*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Case-Control Studies
  • Chromatography, High Pressure Liquid / methods
  • Female
  • Humans
  • Idiopathic Pulmonary Fibrosis / drug therapy*
  • Idiopathic Pulmonary Fibrosis / physiopathology
  • Levofloxacin / pharmacokinetics*
  • Lung / metabolism
  • Lung / physiopathology
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Pulmonary Diffusing Capacity
  • Tandem Mass Spectrometry / methods
  • Tissue Distribution

Substances

  • Anti-Bacterial Agents
  • Levofloxacin