Quantifying the local resolution of cryo-EM density maps

Nat Methods. 2014 Jan;11(1):63-5. doi: 10.1038/nmeth.2727. Epub 2013 Nov 10.


We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Alleles
  • Cryoelectron Microscopy / methods*
  • Fourier Analysis
  • Imaging, Three-Dimensional
  • Likelihood Functions
  • Models, Theoretical
  • Normal Distribution
  • Proteins / chemistry*
  • Ribosomes / chemistry
  • Software
  • Viruses / chemistry


  • Proteins