Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

M Nyman; A Wallin

doi:10.1007/BF00235264

Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

Plant Cell Rep. 1992 Mar;11(2):105-8. doi: 10.1007/BF00235264.

Authors

M Nyman¹, A Wallin

Affiliation

¹ Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden.

PMID: 24213496
DOI: 10.1007/BF00235264

Abstract

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.