Improved Method for the Transformation of Arabidopsis Thaliana With Chimeric Dihydrofolate Reductase Constructs Which Confer Methotrexate Resistance

Plant Cell Rep. 1992 Apr;11(3):118-21. doi: 10.1007/BF00232162.


A modified root explant transformation method has been developed that is effective in producing transgenic Arabidopsis thaliana plants which are methotrexate resistant due to the integration of T-DNA vectors containing a chimeric dihydrofolate reductase gene. Molecular analysis shows that transformed methotrexate resistant plants contain the expected T-DNA construct with the chimeric gene. This transformation method also works well with other plant selectable markers, including hygromycin phosphotransferase and neomycin phosphotransferase II.