Single step BP/LR combined Gateway reactions

Biotechniques. 2013 Nov;55(5):265-8. doi: 10.2144/000114101.

Abstract

The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.

MeSH terms

  • Bacteriophage lambda / genetics*
  • Cloning, Molecular / methods*
  • DNA / genetics*
  • Genetic Vectors / genetics*
  • Plasmids / genetics
  • Recombination, Genetic*

Substances

  • DNA