Solid phase microextraction fills the gap in tissue sampling protocols

Anal Chim Acta. 2013 Nov 25;803:75-81. doi: 10.1016/j.aca.2013.08.031. Epub 2013 Aug 28.


Metabolomics and biomarkers discovery are an integral part of bioanalysis. However, untargeted tissue analysis remains as the bottleneck of such studies due to the invasiveness of sample collection, as well as the laborious and time-consuming sample preparation protocols. In the current study, technology integrating in vivo sampling, sample preparation and global extraction of metabolites--solid phase microextraction was presented and evaluated during liver and lung transplantation in pig model. Sampling approaches, including selection of the probe, transportation, storage conditions and analyte coverage were discussed. The applicability of the method for metabolomics studies was demonstrated during lung transplantation experiments.

Keywords: In vivo SPME; Metabolomics; Surgery; Tissue sampling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Liver / metabolism*
  • Liver Transplantation
  • Lung / metabolism*
  • Lung Transplantation
  • Metabolome
  • Metabolomics / methods*
  • Organ Preservation / methods*
  • Solid Phase Microextraction / methods*
  • Specimen Handling / methods
  • Swine