Presence and distribution of urocortin and corticotrophin-releasing hormone receptors in the bovine thyroid gland

Anat Histol Embryol. 2014 Dec;43(6):429-34. doi: 10.1111/ahe.12094. Epub 2013 Nov 13.


Urocortin (UCN), a 40 amino acid peptide, is a corticotrophin-releasing hormone (CRH)-related peptide. The biological actions of CRH family peptides are mediated via two types of G-protein-coupled receptors, CRH type 1 (CRHR1) and CRH type 2 (CRHR2). The aim of this study was to investigate the expression of UCN, CRHR1 and CRHR2 by immunoprecipitation, Western blot, immunohistochemistry and RT-PCR in the bovine thyroid gland. Immunoprecipitation and Western blot analysis showed that tissue extracts reacted with the anti-UCN, anti-CRHR1 and anti-CRHR2 antibodies. RT-PCR experiments demonstrated that mRNAs of UCN, CRHR1 and CRHR2 were expressed. UCN immunoreactivity (IR) and CRHR2-IR were found in the thyroid follicular and parafollicular cells and CRHR1-IR in the smooth muscle of the blood vessels. These results suggest that a regulatory system exists in the bovine thyroid gland based on UCN, CRHR1 and CRHR2 and that UCN plays a role in the regulation of thyroid physiological functions through an autocrine/paracrine mechanism.

MeSH terms

  • Animals
  • Blotting, Western / veterinary
  • Cattle / physiology*
  • Immunoprecipitation / veterinary
  • Receptors, Corticotropin-Releasing Hormone / genetics
  • Receptors, Corticotropin-Releasing Hormone / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Thyroid Gland / metabolism*
  • Urocortins / genetics
  • Urocortins / metabolism*


  • CRF receptor type 2
  • Receptors, Corticotropin-Releasing Hormone
  • Urocortins
  • CRF receptor type 1

Associated data

  • RefSeq/NM_173979