MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos

Biochem Biophys Res Commun. 2013 Nov 29;441(4):976-81. doi: 10.1016/j.bbrc.2013.11.006. Epub 2013 Nov 9.


MicroRNAs (miRNAs) are non-protein-coding sequences that play a crucial role in tumorigenesis by negatively regulating gene expression. Here, we found that miR-490-5p is down-regulated in human bladder cancer tissue and cell lines compared to normal adjacent tissue and a non-malignant cell line. To better characterize the function of miR-490-5p in bladder cancer, we over-expressed miR-490-5p in bladder cancer cell lines with chemically synthesized mimics. Enforced expression of miR-490-5p in bladder cancer cells significantly inhibited the cell proliferation via G1-phase arrest. Further studies found the decreased c-Fos expression at both mRNA and protein levels and Luciferase reporter assays demonstrated that c-Fos is a direct target of miR-490-5p in bladder cancer. These findings indicate miR-490-5p to be a novel tumor suppressor of bladder cancer cell proliferation through targeting c-Fos.

Keywords: Bladder cancer; Proliferation; c-Fos; miR-490-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Down-Regulation
  • G1 Phase
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Genes, Reporter
  • Genes, Tumor Suppressor / physiology*
  • Humans
  • Luciferases / genetics
  • MicroRNAs / metabolism
  • MicroRNAs / physiology*
  • Proto-Oncogene Proteins c-fos / genetics*
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / pathology*


  • 3' Untranslated Regions
  • MIRN490 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-fos
  • Luciferases