Metabonomics study of the therapeutic mechanism of Gynostemma pentaphyllum and atorvastatin for hyperlipidemia in rats

PLoS One. 2013 Nov 1;8(11):e78731. doi: 10.1371/journal.pone.0078731. eCollection 2013.

Abstract

Gynostemma pentaphyllum (GP) is widely used for the treatment of diseases such as hyperlipidemia, fatty liver and obesity in China, and atorvastatin is broadly used as an anti-hyperlipidemia drug. This research focuses on the plasma and liver metabolites in the following four groups of rats: control, a hyperlipidemia model, a hyperlipidemia model treated with GP and a hyperlipidemia model treated with atorvastatin. Using (1)H-NMR-based metabonomics, we elucidated the therapeutic mechanisms of GP and atorvastatin. Orthogonal Partial Least Squares-Discriminant analysis (OPLS-DA) plotting of the metabolic state and analysis of potential biomarkers in the plasma and liver correlated well with the results of biochemical assays. GP can effectively affect lipid metabolism, and it exerts its anti-hyperlipidemia effect by elevating the level of phosphatidylcholine and decreasing the level of trimethylamine N-oxide (TMAO). In contrast, atorvastatin affects hyperlipidemia mainly during lipid metabolism and protein metabolism in vivo.

MeSH terms

  • Animals
  • Anticholesteremic Agents / pharmacology
  • Atorvastatin
  • Cholesterol / blood
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Gynostemma / chemistry*
  • Heptanoic Acids / pharmacology*
  • Hyperlipidemias / blood
  • Hyperlipidemias / drug therapy*
  • Hyperlipidemias / metabolism
  • Lipid Metabolism / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Magnetic Resonance Spectroscopy
  • Male
  • Metabolomics*
  • Methylamines / metabolism
  • Phosphatidylcholines / metabolism
  • Phytotherapy / methods
  • Plant Extracts / pharmacology*
  • Pyrroles / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Treatment Outcome
  • Triglycerides / blood

Substances

  • Anticholesteremic Agents
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Heptanoic Acids
  • Methylamines
  • Phosphatidylcholines
  • Plant Extracts
  • Pyrroles
  • Triglycerides
  • Cholesterol
  • Atorvastatin
  • trimethyloxamine

Grant support

The authors have no support or funding to report.