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. 2014 Jan;42(Database issue):D1253-60.
doi: 10.1093/nar/gkt1060. Epub 2013 Nov 12.

DNASU Plasmid and PSI:Biology-Materials Repositories: Resources to Accelerate Biological Research

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Free PMC article

DNASU Plasmid and PSI:Biology-Materials Repositories: Resources to Accelerate Biological Research

Catherine Y Seiler et al. Nucleic Acids Res. .
Free PMC article

Abstract

The mission of the DNASU Plasmid Repository is to accelerate research by providing high-quality, annotated plasmid samples and online plasmid resources to the research community through the curated DNASU database, website and repository (http://dnasu.asu.edu or http://dnasu.org). The collection includes plasmids from grant-funded, high-throughput cloning projects performed in our laboratory, plasmids from external researchers, and large collections from consortia such as the ORFeome Collaboration and the NIGMS-funded Protein Structure Initiative: Biology (PSI:Biology). Through DNASU, researchers can search for and access detailed information about each plasmid such as the full length gene insert sequence, vector information, associated publications, and links to external resources that provide additional protein annotations and experimental protocols. Plasmids can be requested directly through the DNASU website. DNASU and the PSI:Biology-Materials Repositories were previously described in the 2010 NAR Database Issue (Cormier, C.Y., Mohr, S.E., Zuo, D., Hu, Y., Rolfs, A., Kramer, J., Taycher, E., Kelley, F., Fiacco, M., Turnbull, G. et al. (2010) Protein Structure Initiative Material Repository: an open shared public resource of structural genomics plasmids for the biological community. Nucleic Acids Res., 38, D743-D749.). In this update we will describe the plasmid collection and highlight the new features in the website redesign, including new browse/search options, plasmid annotations and a dynamic vector mapping feature that was developed in collaboration with LabGenius. Overall, these plasmid resources continue to enable research with the goal of elucidating the role of proteins in both normal biological processes and disease.

Figures

Figure 1.
Figure 1.
Screen shot of the dynamic vector browse feature. Clicking on any of the headings (tag, protease cleavage site or promoter) will sort the list to cluster the vectors by that feature dynamically. Clicking on the vector name will link to vector details pages and ordering information.
Figure 2.
Figure 2.
(A) The Dynamic (DyNA) Vector Map, a widget created by LabGenius. (B) The PDB Structure viewer shows both the 2D and a dynamic 3D view of the protein structure. Selecting different PDB IDs will automatically switch between structures available for that gene.

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