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, 9 (2), 65-8

Production of Theanine and Other γ-Glutamyl Derivatives by Camellia Sinensis Cultured Cells

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Production of Theanine and Other γ-Glutamyl Derivatives by Camellia Sinensis Cultured Cells

Y Orihara et al. Plant Cell Rep.

Abstract

The maximum theanine production by Camellia sinensis cultured cells was achieved by culturing in the modified MS medium containing 2 mg/l indole-3-butyric acid, 0.1 mg/l kinetin, 0 mM NH4NO3 and 39.6 mM KNO3 with 40 mM ethylamine hydrochloride or 20 mM ethylamine hydrochloride and 10 mM L-glutamic acid. Other primary amines, such as methylamine, n-butylamine, 2-hydroxyethylamine, 2cyanoethylamine, aniline, benzylamine and phenylethylamine, were also biotransformed to N(5)-alkyl-L-glutamine derivatives by C. sinensis cultured cells.

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References

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