Neonatal human foreskin keratinocytes produce 1,25-dihydroxyvitamin D3

Biochemistry. 1986 Apr 8;25(7):1545-8. doi: 10.1021/bi00355a013.

Abstract

Primary cultures of neonatal human foreskin keratinocytes converted 25-hydroxyvitamin D in high yield to a metabolite with the chromatographic behavior of 1,25-dihydroxyvitamin D3. The identity of this metabolite as 1,25-dihydroxyvitamin D3 was confirmed both by its potency in displacing 1,25-dihydroxyvitamin D3 in the chick cytosol receptor assay and by mass spectral analysis. These results suggest that 1,25-dihydroxyvitamin D3 may be formed in the epidermis to regulate vitamin D production by the epidermis and to provide an alternative to 1,25-dihydroxyvitamin D3 production by the kidneys.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcifediol / metabolism
  • Calcitriol / biosynthesis*
  • Calcitriol / isolation & purification
  • Calcitriol / metabolism
  • Cells, Cultured
  • Chickens
  • Chromatography, High Pressure Liquid / methods
  • Cytosol / metabolism
  • Epidermis / metabolism*
  • Humans
  • Infant, Newborn
  • Intestinal Mucosa / metabolism
  • Keratins / metabolism*
  • Male
  • Mass Spectrometry
  • Receptors, Calcitriol
  • Receptors, Steroid / metabolism
  • Skin / metabolism*
  • Tritium

Substances

  • Receptors, Calcitriol
  • Receptors, Steroid
  • Tritium
  • Keratins
  • Calcitriol
  • Calcifediol