Abstract
Primary cultures of neonatal human foreskin keratinocytes converted 25-hydroxyvitamin D in high yield to a metabolite with the chromatographic behavior of 1,25-dihydroxyvitamin D3. The identity of this metabolite as 1,25-dihydroxyvitamin D3 was confirmed both by its potency in displacing 1,25-dihydroxyvitamin D3 in the chick cytosol receptor assay and by mass spectral analysis. These results suggest that 1,25-dihydroxyvitamin D3 may be formed in the epidermis to regulate vitamin D production by the epidermis and to provide an alternative to 1,25-dihydroxyvitamin D3 production by the kidneys.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Calcifediol / metabolism
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Calcitriol / biosynthesis*
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Calcitriol / isolation & purification
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Calcitriol / metabolism
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Cells, Cultured
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Chickens
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Chromatography, High Pressure Liquid / methods
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Cytosol / metabolism
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Epidermis / metabolism*
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Humans
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Infant, Newborn
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Intestinal Mucosa / metabolism
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Keratins / metabolism*
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Male
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Mass Spectrometry
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Receptors, Calcitriol
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Receptors, Steroid / metabolism
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Skin / metabolism*
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Tritium
Substances
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Receptors, Calcitriol
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Receptors, Steroid
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Tritium
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Keratins
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Calcitriol
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Calcifediol