Two temperature-sensitive polA mutants: an approach to the role in vivo of DNA polymerase I

Basic Life Sci. 1975;5A:209-11. doi: 10.1007/978-1-4684-2895-7_27.


DNA polymerase I (Pol I) purified from a strain carrying polA12 demonstrates a defect primarily in the polymerase portion of the molecule. The mutant enzyme is altered in its structure and template specificity. Pol I isolated from strains carrying polAexl are primarily defective in 5' in equilibrium 3' exonuclease. The polAexl lesion renders strains conditonally lethal.

MeSH terms

  • DNA Nucleotidyltransferases / metabolism*
  • Drug Stability
  • Escherichia coli / enzymology*
  • Hydrogen-Ion Concentration
  • Mutation
  • Temperature


  • DNA Nucleotidyltransferases