Effects of membrane depolarization and divalent cations on anaphylactic histamine secretion

Cell Calcium. 1986 Apr;7(2):107-14. doi: 10.1016/0143-4160(86)90013-8.


The effects of membrane depolarization and divalent cations on histamine release have been studied in sensitized mast cells. Membrane potential of these cells has been measured with intracellular microelectrodes. Our results show that mast cells have a large resting potential (-61 +/- 12 mV) however they do not generate active membrane electrical responses when are depolarized by passing current through the recording microelectrode. High external K+ does not increase histamine release. Histamine secretion is supported by alkali-earth divalent cations (Ca2+ greater than Sr2+ greater than Ba2+) but strongly inhibited by transition metals. Ca2+ concentrations above 1 mM inhibit histamine release, however, this effect is not mimicked by Sr2+ and Ba2+.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / physiopathology*
  • Animals
  • Barium / pharmacology
  • Calcium / pharmacology
  • Cations, Divalent
  • Female
  • Histamine Release / drug effects*
  • In Vitro Techniques
  • Male
  • Mast Cells / drug effects
  • Mast Cells / metabolism*
  • Mast Cells / physiology
  • Membrane Potentials
  • Rats
  • Rats, Inbred Strains
  • Strontium / pharmacology


  • Cations, Divalent
  • Barium
  • Calcium
  • Strontium